Endospore Staining- Principle, Reagents, Procedure and Result. The endospore stain is a differential stain used to visualize bacterial endospores. Endospore Stain . Spores may be spherical or elliptical. Last Updated on: January 11, 2020 by Sagar Aryal Capsule Stain- Principle, Procedure and Result Interpretation. Allow the smear to only air-dry. Figure 4: Schematic of the Capsule Staining … Within bacteria, endospores are protective structures used to survive extreme conditions, but this protective nature makes them difficult to stain using normal techniques such as simple staining and Gram staining. Principle: Red/pink: vegetative cells. Capsule stain is a type of differential stain which involves the use of two stains; primary stain and the counterstain. Capsule is synthesized in the cytoplasm and secreted to the outside of the cell where it surrounds the bacterium. Learn vocabulary, terms, and more with flashcards, games, and other study tools. The main purpose of endospore staining is to differentiate bacterial spores from other vegetative cells and to differentiate spore formers from non-spore formers. Place a single drop of India ink on a clean microscope slide, adjacent to the frosted adge. A capsule in bacteria is the result of amorphous viscid secretion released by the bacteria. Is a gelatinous outer layer that is secreted by the cell and that surrounds and adheres to the cell wall. Capsule stain is a type of differential stain which uses acidic and basic dyes to stain background & bacterial cells respectively so that presence of capsule is easily visualized. With this staining procedure, the vegetative bacteria stain blue and the endospores are colorless. With this staining procedure, the vegetative bacteria stain blue … Capsules appear clear or … When this secretion remains loose and un-demarcated it is called a slime layer and when it is organized into a sharply defined structure it is called as Capsule. Endospores are formed by a few genera of bacteria, such as Bacillus . Do not heat-fix as this will cause the capsule to shrink or be destroyed. spore stain The endospore stain is a differential stain used to visualize bacterial endospores. Spores may be located in the middle of the cell, at the end of the cell, or between the end and middle of the cell. INTRODUCTION TO CAPSULE STAINING TECHNIQUE. Staining • Staining -It’s a process of adding a dye to a microbial culture. Spore Staining & Capsule Staining. The spores retain the green of the primary stain. A capsule in bacteria is the result of amorphous viscid secretion released by the bacteria. Stain Protocols – BIOL 2420 Smith – 2010 Page 3 of 4 Capsule Stain 1. 11. Common diseases caused by encapsulated bacteria include pneumonia, meningitis, and salmonellosis. Principle of Spore Staining: A differential staining technique (the Schaeffer-Fulton method) is used to distinguish between the vegetative cells and the endospores. By forming spores, bacteria can survive in hostile conditions. The polymers which make up the capsule tend to be uncharged and as such they are not easily stained. For this reason we use a NEGATIVE STAIN to visualize them. As a result, acidic and basic stains will often fail to adhere on to them. Allow smear to air-dry, and heat fix in the usual manner. In 1933, the procedure was modified by Shaeffer and Fulton. OBJECT: To identify the organism in a given sample REQUIREMENTS: Sample, glass slide, wire loop, burner, malachite green, safranine, distilled water and cedar wood oil, and a microscope.

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